Integrated Solutions for Western Blot Incubation, Blocking and Imaging from WIX TECHNOLOGY

The comparison between WIX-autoPP and manual incubation (shakers) with room temperature

200mA, 1.5h, wet transfer, two membranes, one membrane incubated by manual incubation with room temperature, while the other incubated by WIX-autoPP.

Left: the result by WIX-autoPP

Right: the result of manual incubation

Left: WIX-SKL180–LCD

Right: WIX-SKL330–LCD

Currently,  WIX Technology has six models of shakers for selection,

WIX-SKO330/LCD NC Circular Shaker

WIX-SKO180/LCD NC Circular Shaker

WIX-SKL330/LCD NC Linear Shaker

WIX-SKL180/LCD NC Linear Shaker

WIX-SKR330/LCD NC Tilting Shaker

WIX-SKR180/LCD NC Tilting Shaker

WIX-SKD330/LCD NC 3D Shaker

WIX-SKD180/LCD NC 3D Shaker

Mammary Factor (90kDa)

Left: the result by manual incubation

Right: the result of WIX-autoPP

β-Actin Loading Control, both results by WIX-autoPP

Wet transfer: 250mA, 1.5h; semi-dry transfer: 1.3A, 15min

Left: semi-dry transfer

Right: wet transfer

Left: miniblot

Right: WIX-miniBLOT4

Wet transfer, WIX TECHNOLOGY has two models for your choice, 2-gel wet transfer (mini vertical blot) WIX-miniBLOT (the max transfer size is 100x75mm), and 4-gel wet transfer WIX-miniBLOT4 (the max transfer size is 80x85mm).  

Semi-dry transfer, WIX-fastBLOT is highly recommended, blot size is 15.5×11×2 cm. Two cassettes, high dissipation, transfer 4 mini gels or 2 midi gels simultaneously around 15 mins.

Imaging results

Conditions: two 12% spacer gels, two wells with sample 5μ.

Fast transfer: 50V, 25min. Two membranes were exposed simultaneously.

GAPDH antibody.

Left: manual incubation

Right: WIX-autoPP incubation

The results: minor differences, WIX-autoPP can replace manual incubation, helping saving your time, can incubate at most 24 pcs of membranes simultaneously.

Imaging result

Marker 

Sample

Merged imaging

The imaging results were by WIX chemiluminiscence WIX-chemiPHOTO.

The advantages of WIX-fastBLOT, WIX-autoPP and WIX-chemiPHOTO

WIX-fastBLOT

• Consumables: 5× rapid semi-dry transfer buffer, semi-dry transfer filter paper
• The WIX 5× rapid semi-dry transfer membrane buffer uses a unique formula and the semi-dry transfer method, enabling efficient and rapid protein transfer onto the blotting membrane (PVDF membrane or NC membrane) within 10-20 minutes.
• Easy use: No need for transfer tank, transfer clips, etc. The “sandwich” only needs to placed in the cassettes with uniformity.
• Environmentally friendly: The rapid transfer buffer can use ethanol instead of methanol, reducing harm to the experimenter and the environment.
• Good compatibility: The rapid transfer buffer is compatible with various gels such as Tris-glycine gel, Bis-Tris gel, etc.
• Time saving: The rapid transfer buffer can complete protein transfer within 10-20 minutes.
• Less buffer usage: Only 10-20 ml of 1× rapid semi-dry transfer membrane buffer is needed for one piece of gel.

WIX-autoPP

• Easy use, the step from blocking to incubation can be finished automatically.
• Test temperature and test conditions can be set upon your needs.
• Various sizes of incubation tanks can meet your demand for different sizes of membranes.
• No need for manual operation, the repeatability of imaging results is well.
• No need for extra consumables, same as manual incubation.

WIX-chemiPHOTO

• Easy use: The exposure time can be automatically adjusted according to the brightness of the sample, without any human intervention.
• Built-in computer: The computer and imaging system are designed as a single unit, eliminating the need for an additional computer.
• It features both fully automatic imaging and manual imaging modes, enabling the capture of extremely low-level signals.