Western Blot Transfer Systems
Achieve highly efficient and uniform protein transfers with WIX Technology’s blot transfer systems. Engineered specifically for Western blotting applications, our transfer instruments ensure the precise migration of proteins from polyacrylamide gels to nitrocellulose or PVDF membranes. Whether you need, have the demand of reliable wet transfer tanks for large-molecular-weight proteins or rapid semi-dry blotting systems for high-throughput workflows, browse our selection below to optimize your downstream protein analysis.
High-Efficiency Western Blot Transfer Solutions
In proteomics, a flawless Western blot depends entirely on a complete and uniform transfer of proteins from your SDS-PAGE gel to the membrane. As a specialized manufacturer of life science instruments, WIX Technology designs blot transfer systems that deliver high-resolution results. Manufactured from durable, chemical-resistant polycarbonate, our transfer instruments provide the structural integrity and electrical consistency required for the most demanding molecular biology laboratories.
Advanced Features of WIX Blotting Instruments
Wet transfer:
High-current fast transfer and low-current overnight transfer are available.
Built-in cooling units absorb heat efficiently.
Fast semi-dry transfer:
With high efficient heat dissipation channel, to release the heat during the operation and ensure the reliability of the lab in the low temperature.
Easy operation: compatible with ready-to-use blot consumable package of other brands, no need to prepare buffer and membrane.
WIX-coolBLOT:
Built-in power supply and thermostatic circulator to cycle buffer directly. Output of voltage and current in the same time, the buffer temperature is quickly reduced simultaneously to avoid the failure of the experiment due to high temperature. The power supply function is used for protein electrophoresis or nucleic acid electrophoresis.
Wet Transfer Tanks vs. Semi-dry Blotting
WIX offers versatile solutions tailored to your specific experimental needs:
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Wet Transfer Systems (Tank Blotters): The gold standard for Western blotting. Ideal for transferring large proteins (>100 kDa) and achieving the highest sensitivity. Accommodates 1-4 gel cassettes simultaneously for increased throughput.
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Semi-Dry & Fast Blotters: Perfect for smaller proteins and rapid results. These systems drastically reduce transfer times and require a minimal volume of transfer buffer, making them highly efficient and cost-effective for routine screening.
Frequently Asked Questions (FAQ)
Q: How do I prevent my transfer buffer from overheating during a 100V wet transfer?
A: Our wet blot transfer systems include a sealed ice cooling core (cooling block). Simply pre-freeze the core and insert it into the tank alongside your transfer cassettes. We also recommend pre-chilling your transfer buffer to maintain optimal temperatures throughout the run.
Q: Can I transfer multiple gels at the same time?
A: Yes. Our standard wet transfer tanks are designed to accommodate multiple transfer cassettes simultaneously. Depending on the model, you can easily transfer 2 to 4 mini-gels in a single run, significantly improving your lab’s throughput.
Q: What type of membranes are compatible with your transfer systems?
A: Our electroblotters provide uniform contact pressure suitable for all standard blotting membranes, including PVDF, Nitrocellulose (NC), and Nylon membranes, regardless of the pore size (0.22 µm or 0.45 µm).
