The introduction of western blot protein transfer has long been hindered by traditional wet transfer methods, which suffer from lengthy cycles (60–90 min), high buffer consumption, poor thermal stability, and limited throughput. These limitations impair the reproducibility and efficiency of experiments, especially in the high-demand field of proteomics research.The Fast Semi-dry Blot System WIX-fastBLOT directly addresses these pain points with an innovative thinlayer buffer design, independent dualcassette configuration, and integrated heat dissipation. The system can complete the transfer of 2 mini gels within 10 minutes and 4 mini gels or 2 midi gels within 15 minutes, reducing waste of consumables.